[BBF Standards] Experimental standards discussion

[julius.lucks]

>

Hi List,


> 1. How to get people to use Jason Kelly's promoter characterization  
> standard
>

Towards this end, Jason and I have discussed making a modification to  
the standard (or maybe the next version of the standard) to expand  
the strain used from Top10 to any lab strain used for experiments.   
The idea would be to start from purified plasmids instead of already- 
transformed strains, and add a transformation step to the protocol  
where you put the plasmids into the strain that you do experiments with.

I think this proposed modification of the standard addresses a  
practicality issue in the first version for labs that do not use  
Top10 cells - forcing them to do so to comply with the standard might  
make things awkward because of the different doubling times between  
Top10 and the strain that they would use for the rest of the  
experiment.  Currently we are using just blank cells to normalize our  
measurement, and transforming with a standardized fluorescence  
plasmid would be a much better way to perform the normalization.   
(Credit to John Dueber, Weston Whitaker and Stanley Qi for bringing  
up this point in a chat in the lab.)

To kick things off, we (Arkin lab, Potter St., Berkeley) are going to  
do the measurements on Tg1 and DH10B cells.  If anyone else is  
interested in this, contact Jason or I and we can send you some  
plasmids.  Ideally we could gather a list of all the measurements  
done in a variety of the most popular strains to provide a master  
reference list as part of the next standard revision.

Cheers,

Julius

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