[BBF Standards] functional composition of BioBrick parts?

[Caroline Ajo-Franklin]

Hi Drew & Members of the Standards working group,
  At the November workshop, Alistair Elfick, Christopher Mason, Barry
Canton, Reshma Shetty, Kim de Mora, Matthieu Buprelle, Vincent
Rouilly, Scott Mohr, and myself met and outlined two initial goals for
our working group:
1) to determine the variability of in vivo fluorescence measurements
of identical strains across different labs
2) to draft a standard for transcriptional devices
 While this was an excellent start, we haven't yet gotten farther. We
are eager to have something in hand for the March 31 meeting, so your
reminder is quite welcome.

Standards working group, could I please get some volunteers to help
who have access to a FACS machine and would be interested in making a
handful of in vivo fluorescence measurements before the March 31st
meeting? This experiment is designed to answer the question: How much
does the relative fluorescence of two (or three) GFP-expressing E.coli
strains vary between different labs assuming identical media & growth
conditions? It would be great to get measurements from several labs
spread across the world.
 Could I also get some volunteers interested in drafting a standard
sheet for transcriptional devices to be presented at the March 31st
meeting? The goal here is to articulate the minimum description of a
transcriptional device which would enable its use in a higher order
device, and would build upon substantial starts present in the
Registry documentation and in the literature.

 If you interested in joining the struggle to standardize biological
parts through either of these efforts, please drop me an e-mail by
this Sunday Feb 2.

Best-
Caroline

On Jan 27, 2008 3:53 PM, Drew Endy <endy at mit.edu> wrote:
>
> Hi Caroline, Chris, Deepak,
>
>
> The Technical Standards Group at the BBF recently launched.
>
> A number of new folks have signed up and are anxious to help.
>
> At the November BBF workshop we expressed an interest in working together on
> standard(s) that define how BioBrick parts function so that, when two parts
> are combined, the resulting behavior of the composite part is as expected,
> and not some surprising emergent property.  Has there been any progress?
> Who is leading our discussion?  Can we get a report / update?  Should we
> recruit more people to help with this topic?  E.g., I know that Reshma
> Shetty and Jason Kelly have been working on a PoPS level standard.
>
> The next workshop is coming up on March 1 at UCSF and we're hoping to be
> able to discuss progress and plans to continue to take things forward.
>
> If you haven't signed up for the mailing list, here's the URL to do so:
>
> Standards mailing list
> Standards at biobricks.org
> http://biobricks.org/mailman/listinfo/standards_biobricks.org
>
> Thanks / be great!
> Drew
>
>
> Begin forwarded message:
>
> From: "Julius B. Lucks" <julius at younglucks.com>
> Date: January 27, 2008 5:11:56 PM EST
> To: standards at biobricks.org
> Subject: [BBF Standards] Starting off discussions
>
> Maybe a good place to start discussions is for the 4 topic leaders to
> send something to the list saying a little bit about the topic, and
> what needs to get done before March 1.  That would be very helpful
> for those of us new to the discussions to figure out where we can
> jump in and help out.
>
>
> A small number of groups set out to start working on some of these,
> and report back at the next standards workshop (March 1 at UCSF).
> Here are the four sub groups that got started:
>
> Physical Assembly of BioBrick Parts:
> Randy Rettberg, Stephen Davies, Austin Che, Kristina Muller, Raik
> Grunberg, Chris Anderson, John Dueber, Scott Mohr
>
> Push / Pull Parts Across Computer Networks:
> Raik Grunberg, Drew Endy, Christopher Mason, Randy Rettberg, Chris
> Anderson, John Dueber, Deepak Chandran, Mac Cowell, Sean Sleight, Matt
> Silver
>
> Functional Composition:
> Caroline Ajo-Franklin, Drew Endy, Christopher Mason, Deepak Chandran,
>
> Experiments & Results:
> Chris Anderson, Kristian Muller, Drew Endy, John Dueber
>
> The first person listed above for each group was supposed to be the
> leader of the group.  We'll have to check with them to see what they
> have been doing, and find new leaders if needed.
>
>
>
> Cheers,
>
> Julius
>
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> ----------------
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> ------------------------------------------------------------------------
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>
>
> _______________________________________________
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>
>



-- 
Caroline Ajo-Franklin, Ph.D.
Staff Scientist, Biological Nanostructures Facility
The Molecular Foundry
Lawrence Berkeley National Laboratory
phone: 510.486.4299
e-mail: CAjo-Franklin at lbl.gov