[BBF Standards] Biobrick BAC?

[Reshma Shetty]

I believe that one of the largest parts in the registry is ~5.6kb
(http://partsregistry.org/wiki/index.php?title=Part:BBa_I6001)

I briefly looked into making a BioBrick BAC vector.  The problem is
that the full-length F plasmid has several BioBrick sites in it that
need to be removed.  There are smaller variants of the F plasmid that
don't have as many BioBrick sites it so there might be something
available with a partial BioBrick cloning site.

I have a vague recollection that Chris Anderson has a BAC with a
partial BioBrick cloning site, but my information is probably two
years old.  (Apologies if Chris already responded off-list.)

-Reshma

On Thu, Jul 24, 2008 at 12:48 PM, Drew Endy <endy at mit.edu> wrote:
> Apologies for a slightly off topic post.
>
> What's the biggest insert anybody's put into a BioBrick vector?  Any
> validated bacterial artificial chromosomes, per chance?
>
> Thanks!
>
> Drew
>
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