[BBF Standards] Biobrick BAC?

[Randy Rettberg]

Drew,

Looking in the database for long parts with status "Available":

  Chris Anderson has BBa_J61031 "Amplifiable BAC plasmid"

BBa_I766212 is a composite part of length 8270bp. It seems to have  
been built
and tested, but did not work correctly.

Randy




On Jul 24, 2008, at 3:10 PM, Reshma Shetty wrote:

> I believe that one of the largest parts in the registry is ~5.6kb
> (http://partsregistry.org/wiki/index.php?title=Part:BBa_I6001)
>
> I briefly looked into making a BioBrick BAC vector.  The problem is
> that the full-length F plasmid has several BioBrick sites in it that
> need to be removed.  There are smaller variants of the F plasmid that
> don't have as many BioBrick sites it so there might be something
> available with a partial BioBrick cloning site.
>
> I have a vague recollection that Chris Anderson has a BAC with a
> partial BioBrick cloning site, but my information is probably two
> years old.  (Apologies if Chris already responded off-list.)
>
> -Reshma
>
> On Thu, Jul 24, 2008 at 12:48 PM, Drew Endy <endy at mit.edu> wrote:
>> Apologies for a slightly off topic post.
>>
>> What's the biggest insert anybody's put into a BioBrick vector?  Any
>> validated bacterial artificial chromosomes, per chance?
>>
>> Thanks!
>>
>> Drew
>>
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>
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