[BBF Standards] How do I get a RFC (request for comment) number for a new standard?

[Drew Endy]

Hi John,

The technical standards process is detailed online here:
http://openwetware.org/wiki/The_BioBricks_Foundation:Standards/Technical#BBF_Technical_Standards_Setting_Process

It sounds to me like you might want to coordinate w/ Jason.  He's not  
yet requested a RFC (as I understand it, he is awaiting more results  
from other groups using and validating his *reference* standard).  One  
option would be to merge efforts.  Another option would be to take the  
dual luciferase system forward as its own standard (have you worked  
with another group, outside of Brown, to validate your proposed  
solution).

Hope this helps / let me know!
Drew


On Mar 6, 2008, at 2:03 AM, John Cumbers wrote:

> Hi,
> How do I get a RFC (request for comment) number for a new standard?   
> I have a way to measure promoter-RBS combinations that would  
> complement Jason Kelly's, but would allow for better measurement of  
> weak promoters.  E.g I20259 doesn't show up on Jason's FACS  assay, (http://openwetware.org/wiki/The_BioBricks_Foundation:Standards/Technical/Measurement 
> ) but Jason has said he saw fluorescence under the scope.
>
> The proposed assay uses the dual luciferase system (suggested to me  
> by Tom Knight) which has no background, unlike GFP and should be  
> more accurate for weaker promoters.
>
> Cheers,
> John
>
>
>
> -- 
> John Cumbers,  Graduate Student
> Molecular Biology, Cell Biology, and Biochemistry
> Biology and Medicine, Brown University, Box G-W
> Providence, Rhode Island, 02912, USA
> Tel USA: +1 401 523 8190,  Fax: +1 401 863-2166, UK to USA: 0207 617  
> 7824
>
> Delivery address:  Brown University (EEB) Biomed Stock Room
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