[BBF Standards] How do I get a RFC (request for comment) number for a new standard?

[John Cumbers]

Great, I'll speak with Jason about the possibility of merging the two, in
the meantime, if anyone has a luminometer and would like to run the assay at
their location, please drop me a mail.  You can purchase the dual luciferase
substrates from promega, or I can send you some,
cheers,
John



On Thu, Mar 6, 2008 at 9:00 AM, Drew Endy <endy at mit.edu> wrote:

> Hi John,
> The technical standards process is detailed online here:
>
> http://openwetware.org/wiki/The_BioBricks_Foundation:Standards/Technical#BBF_Technical_Standards_Setting_Process
>
> It sounds to me like you might want to coordinate w/ Jason.  He's not yet
> requested a RFC (as I understand it, he is awaiting more results from other
> groups using and validating his *reference* standard).  One option would be
> to merge efforts.  Another option would be to take the dual luciferase
> system forward as its own standard (have you worked with another group,
> outside of Brown, to validate your proposed solution).
>
> Hope this helps / let me know!
> Drew
>
>
> On Mar 6, 2008, at 2:03 AM, John Cumbers wrote:
>
> Hi,
> How do I get a RFC (request for comment) number for a new standard?  I
> have a way to measure promoter-RBS combinations that would complement Jason
> Kelly's, but would allow for better measurement of weak promoters.  E.gI20259 doesn't show up on Jason's FACS  assay, (
> http://openwetware.org/wiki/The_BioBricks_Foundation:Standards/Technical/Measurement)
> but Jason has said he saw fluorescence under the scope.
>
> The proposed assay uses the dual luciferase system (suggested to me by Tom
> Knight) which has no background, unlike GFP and should be more accurate for
> weaker promoters.
>
> Cheers,
> John
>
>
>
> --
> John Cumbers,  Graduate Student
> Molecular Biology, Cell Biology, and Biochemistry
> Biology and Medicine, Brown University, Box G-W
> Providence, Rhode Island, 02912, USA
> Tel USA: +1 401 523 8190,  Fax: +1 401 863-2166, UK to USA: 0207 617 7824
>
> Delivery address:  Brown University (EEB) Biomed Stock Room
> 34 OLIVE ST, Providence, RI 02912
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>
>
>


-- 
John Cumbers,  Graduate Student
Molecular Biology, Cell Biology, and Biochemistry
Biology and Medicine, Brown University, Box G-W
Providence, Rhode Island, 02912, USA
Tel USA: +1 401 523 8190,  Fax: +1 401 863-2166, UK to USA: 0207 617 7824

Delivery address:  Brown University (EEB) Biomed Stock Room
34 OLIVE ST, Providence, RI 02912
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