[BBF Standards] individual BioHacker

mantese at msn.com mantese at msn.com
Sun Mar 9 13:39:26 EDT 2008


Hi.  I dont' have access to wet-lab space and would like to try to setup something in my
apartment to do some BioBrick work. I'd appreciate any thoughts on how I should start going
about this. 
 
Looking through the protocols, these are some of the necessary items that I've identified:
pipettes
Petri dishes
Agar
PCR kit (those are available commercially)
E. Coli
specific enzymes found on the "Materials section" of Biobricks.org
 
Several questions:
1. I don't want to have E.Coli around, are there some interesting yeast experiments?
 
2. There are very many materials (restriction enzymes, buffers, etc) listed under the "Materials section". 
Can these be paired down to the beginner's working essentials?
 
3. As an aside, I work (full-time at a startup) and live in the SF Bay Area. If there might be a way 
I could collaborate with someone, or at least see how a real lab is setup, it would be very much 
appreciated. 
 
Apologies if I sent an email to the incorrect forum. Take care and thanks much! 
Lucy
 
 
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