[BBF Standards] how to remove the unnecessary protein

[Drew Endy]

John,

Probably a better question for the discuss at syntheticbiology list.

But, you can add tags to proteins that will cause them to be degraded  
*inside* the cell.  In E.coli these tags are recognized by the ssrA  
system.

Drew


On Mar 10, 2008, at 1:06 PM, JUNLING HUO wrote:

> Hi there,
>
> Genetic switches are always made based on gene control in synthetic  
> biology and certain protein concentrations are always used to  
> represent status (on/off) of the switch. However, the signal  
> proteins continue to stay in the media since they generally have  
> longer lifetimes. The existence of these proteins will increase the  
> background noises, which is not what we want.
>
> Does anyone know how to remove these unnecessary protein from the  
> media?
>
> Thanks!
>
> John
>
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