[BBF Standards] question on PoPS devices

[Reshma Shetty]

Hey Deepak,

On Wed, May 21, 2008 at 1:06 PM, Deepak Chandran
<deepakc at u.washington.edu> wrote:

> A PoPS device can only have one input and one output -- is that correct?

No.  PoPS devices can have multiple inputs and/or multiple outputs.
For example, an inverter can have multiple outputs simply by having
multiple copies of the cognate promoter regulating different
downstream devices.  To engineer a device with multiple inputs, simply
use multiple copies of the RBS, coding region and terminator under the
control of different upstream devices.  Stated differently, PoPS
devices do not need to be on contiguous pieces of DNA.

If
> so, is it possible to create a network similar to natural regulatory
> networks (i.e. with one gene regulating multiple genes) solely through the
> use of the PoPS interface?

Sure.  See above.

Let me know if I am unclear on the above.

Thanks,

Reshma