[BBF Standards] question on PoPS devices

[Anil Wipat]

Hi all,

I've been following this with interest. Summarising this I think there
is a difference between PoPS devices that interface 'cis' i.e. next to
each on a contiguous sequence and those that regulate in 'trans' e.g. a
transcription factor that modulates the expression of a target promoter
elsewhere in the genome? Cross talk is going to happen in trans (e.g. a
TF may have multiple targets) whereas PoPs can potentially read through
a device contiguous on the same sequence? 

Cheers

Anil

>-----Original Message-----
>From: standards-bounces at biobricks.org 
>[mailto:standards-bounces at biobricks.org] On Behalf Of Reshma Shetty
>Sent: 21 May 2008 19:43
>To: Deepak Chandran
>Cc: standards at biobricks.org
>Subject: Re: [BBF Standards] question on PoPS devices
>
>Hey Deepak,
>
>On Wed, May 21, 2008 at 1:06 PM, Deepak Chandran 
><deepakc at u.washington.edu> wrote:
>
>> A PoPS device can only have one input and one output -- is 
>that correct?
>
>No.  PoPS devices can have multiple inputs and/or multiple outputs.
>For example, an inverter can have multiple outputs simply by 
>having multiple copies of the cognate promoter regulating 
>different downstream devices.  To engineer a device with 
>multiple inputs, simply use multiple copies of the RBS, coding 
>region and terminator under the control of different upstream 
>devices.  Stated differently, PoPS devices do not need to be 
>on contiguous pieces of DNA.
>
>If
>> so, is it possible to create a network similar to natural regulatory 
>> networks (i.e. with one gene regulating multiple genes) 
>solely through 
>> the use of the PoPS interface?
>
>Sure.  See above.
>
>Let me know if I am unclear on the above.
>
>Thanks,
>
>Reshma
>
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